Correlation analysis showed that TMEM59L expression negatively correlated with activated CD4 T cells and CD8 T cells in most cancer types, and further IPS analysis also replicated the same trend; TMEM59L expression was negatively related to IPS score, AZ, and ECs, while being positively associated with SCs, indicating that TMEM59L could play a key role in the immunosuppressive microenvironment. This evidence concerns the gene TMEM59L and cancer.