R293H/C alteration in the Pkinase domain of PLK1 protein, which was discovered in 2 cases of COAD, 1 case of LUAD, 1 case of ESCA and 1 case of HNSC, can lead to missense mutation of the PLK1 gene, translating from R (Arginine) to H (Histidine) or C (Cysteine) at the site 293 of PLK1 protein, and changing the structure of PLK1 protein subsequently (Supplementary Figure 8B). This evidence concerns the gene PLK1 and colon adenocarcinoma.