Differential genes screened by differential analysis between subgroups (|logFC|> 1 and P <0.05) for univariate cox analysis (P <0.05) to screen three prognostic genes (CCL2, MCF2L and SHB) in tumor cell-enriched region and four prognostic genes (FAM169B, KRT27, NT5M and TIMP1) in immune cell-enriched region (Figures 5B, C, S1B, S1C, S1E). This evidence concerns the gene TIMP1 and neoplasm.