In addition, TRAM2 was not only involved in osteosarcoma progression but also closely related to OS, PFS, DSS, TMD, MSI, immune cell infiltration and immune checkpoints in pan-cancer, suggesting that the GCSRGs signature and the target genes in the signature have the potential to serve as the prognostic indicators for a wide range of cancers. This evidence concerns the gene TRAM2 and osteosarcoma.