According to the multivariate analysis (Table 3), LR-M category (odds ratio [OR], 5.60; p = 0.054), marked hepatobiliary phase hypointensity (OR, 9.98; P < 0.001), and serum AFP level > 400 ng/mL (OR, 3.58; p = 0.021) were incorporated into the HCC differentiation score and are illustrated in Fig. 2. This evidence concerns the gene AFP and hepatocellular carcinoma.