The analysis of the EV sub-populations showed that EpCAM+ EVs, which are EVs of epithelial origin, CD45+ leukocyte-derived EVs, and CD41a+ and CD41a+ Annexin V+ platelet-derived EVs, as well as CD31+ endothelium-derived EVs, were significantly increased in celiac disease patients compared with healthy subjects. This evidence concerns the gene ANXA5 and celiac disease.