Excessive fat intake increases the amount of free fatty acid in the blood, increases the risk of obesity, and causes insulin resistance; the mechanism might include the generation of lipid metabolites (diacylglycerol), proinflammatory cytokines (TNF-α, IL1β, IL6, MCP1), and cellular stress, including oxidative and endoplasmic reticulum stress [42]. The gene discussed is TNF; the disease is Obesity.