Using the Western blot approach, cleavage of PARP and caspase 3 in treated cells, expression analysis of apoptotic (P53 and BAX) and anti-apoptotic (BCL-2 and NF-κB) proteins was evaluated to uncover the potential molecular mechanism of the antiproliferation of cancer cell colonies in the MTT assay (Figure 4A). The gene discussed is CASP3; the disease is cancer.