The levels of immunostimulatory cytokines, IL-23, IL-12, TNF-α, and IFN-γ, were remarkably increased, while the immunoinhibitory cytokines, IL-4, IL-13, IL-10, and TGF-β, were decreased when HCC-bearing mice were administrated with bufalin. The gene discussed is TNF; the disease is hepatocellular carcinoma.