Hence, the protein level and activity of NEP was examined in six cancer cell lines, i.e., SiHa and CaSki (primary and metastatic cervical cancer, respectively); SW948 and SW620 (primary and metastatic colon cancer, respectively); HOS and Saos-2 (primary highly and moderately aggressive osteosarcoma) [30], and in normal epithelial cells of the intestine CCD 841 CoTr and osteoblasts hFOB after AKG treatment to determine the potential influence of AKG on NEP expression. The gene discussed is MME; the disease is osteosarcoma.