ANXA5 and breast cancer: Since ABQ-3 displayed notable anticancer effects on CRC and breast cancer cells, we further searched for its apoptotic effects in HCT-116 and MCF-7 cells using the annexin V/ethidium homodimer III staining assay, which was detected by a fluorescence microscope representing apoptosis, necrosis, or late apoptosis/necrosis with green, yellow and red staining, respectively (Figure 6A).