In this study, we first described the presentation and prognosis of RPS24 in HCC and found that RPS24 can regulate HCC proliferation and immune infiltration in the tumor microenvironment, which might affect some signaling pathways, such as the E2F targets, G2M checkpoint, Wnt/β-catenin signaling pathway, and interferon-alpha/gamma. This evidence concerns the gene RPS24 and neoplasm.