Next, we tested the expression of the RPS24 protein in 86 pairs of HCC and adjacent non-tumor tissues (cohort 2) using IHC and showed that RPS24 proteins have different degrees of staining in the cytoplasm, and the protein expression of RPS24 in HCC was significantly higher than that in the non-tumor tissues (Figure 9A, Table 2), suggesting that RPS24 has a cancer-promoting effect on the cytoplasm of HCC cells. This evidence concerns the gene RPS24 and cancer.