A subgroup analysis showed that the overexpression of RPS24 significantly affected the OS of HCC patients aged ≤ 60 years (p = 0.021) with pathological stages III and IV (p = 0.012), M0 (p = 0.003), N0 (p = 0.033), G1 grade (p = 0.037), and a fibrosis ishak score of 0&1/2 (p < 0.001) (Figure 3B–G). This evidence concerns the gene RPS24 and hepatocellular carcinoma.