After treatment, the Lee’s index of rats in the obesity model (OM) + P2X4 shRNA group and the OM + imperatorin group decreased significantly, while the Lee’s index of the OM + NC shRNA group experienced no significant change compared with the OM group (Figure 2B) (p < 0.05). Here, P2RX4 is linked to obesity due to melanocortin 4 receptor deficiency.