We used a sequential, multiplex staining protocol [26] (Supplementary Figure S3a) in which six markers were analyzed simultaneously: CD15 (neutrophil marker), pan-cytokeratin (CK; tumor marker), HIF-1α, GLUT1 and HK2 (glycolytic markers), and DAPI (nuclear marker) (Figure 3a). Here, HIF1A is linked to neoplasm.