GAD2 and Anxiety: In OFT, time spent (Figure 7E, t = 2.856, p = 0.0189), distance traveled in the center area (Figure 7F, t = 2.754, p = 0.0223) as well as entries to the center area (Figure 7G, t = 3.092, p = 0.0129) of hM3Dq group was less than mCherry group, whereas the freezing time (Figure 7H, t = 2.828, p = 0.0198) and immobile time (Figure 7I, t = 2.706, p = 0.0241) was increased compared to the mCherry group, which indicated that pharmacogenetic activation of vLS GAD2 aggravated anxiety-like behavior induced by stress.