Targeted editing reads were detected in all GBM cell lines, and the editing rates were 3.6–6.3% (U-251 MG: 4.9%, KNS-42: 3.6%, SF126: 6.3%, YKG-1: 4.2%; without cell sorting), suggesting that the Cas9 RNP complex electroporation method would be sufficient for single-nucleotide editing in GBM cells. Here, RNPC3 is linked to glioblastoma.