Confirmed by bioinformatics analysis, involvement of CD151,Tspan8 tetraspanins, and genes controlled by miR-24-3p and miR-101 in signaling pathways, which are crucial for carcinogenesis, demonstrates that these tetraspanins and microRNAs are potential biomarkers in assessing the prognosis of metastasis and tumor drug resistance, as well as to select new targets for targeted therapy. Here, TSPAN8 is linked to neoplasm.