In this context, we anticipate mitigation of DMD disease induced-iNOS, and cytokine levels of TNF-α, IL1β, Nox4, NPT, M1 macrophages, MMP9, and the increase in CD4+, and CD8+ cells, diaphragm atrophy, and alveolar leakage in the DMD disease mice treated with iNOS inhibitor. This evidence concerns the gene NOX4 and Duchenne muscular dystrophy.