As depicted by the quantitative analysis, diabetes caused a reduction in the number of bNOS expressing retinal amacrine cells, about 27% compared to Control retinas (Control: n = 12, 100.00 ± 6.48; diabetic untreated: n = 13, 73.47 ± 10.80, **** p < 0.0001 compared to Control) (Figure 3B). Here, NOS1 is linked to diabetes mellitus.