ACSL4 and neoplasm: Concerning the ferroptosis hallmarks, the mRNA of the anti-ferroptosis transcription factor NRF2 and the protein level of GPX4 were downregulated, and the pro-ferroptotic acyl-CoA synthetase long-chain family member 4 (Acsl4) was up-regulated by the LSAA/HPUFA diet in tumor masses (Figure 4d,e), indicating an impaired antioxidant response and increased incorporation of peroxidizable lipids in cell membranes.