In light of the results obtained in vitro and in order to induce ferroptosis in vivo, in BC-bearing mice, we used a combined approach that we previously suggested to be promising for anti-cancer therapy based on the metabolic characteristics of cancer cells [19]: (i) we restricted SAAs in order to reduce the efficiency of GSH/GPX4 system; (ii) we increased the level of dietary PUFAs to raise the amount of peroxidizable lipids. The gene discussed is GPX4; the disease is breast cancer.