It is important to note that, though both CGL1 and CGL2 showed most sensitive to DLD1 through the three types of colorectal cancer cells in vitro, their anti-cancer effect on HCT116 tumors were actually more obvious than on DLD1 tumors in vivo, like many agents having different anti-cancer effects between in vitro and in vivo. The gene discussed is GZMH; the disease is cancer.