The degree of microphthalmia was paralleled by EtOH dose-dependent reductions in eye and brain BDNF levels (ELISA) and PMP22 expression (FACS), and by dramatic increases in brain (FACS; Figure 4C), and eye caspase-3 activity (FACS; Figure 4D). The gene discussed is BDNF; the disease is microphthalmia.