The transcriptome analysis of tumor biopsies from patients treated with anti-PD-1 monotherapy (nivolumab) or in combination anti-PD1 plus anti-CTLA-4 therapy (ipilimumab) correlated well with the in vitro analysis of gene expression signatures of melanoma cell lines following exposure to interferon-γ [93]. This evidence concerns the gene CTLA4 and neoplasm.