CTLA4 and cancer: A combination of immunohistochemical (IHC) analysis with RNA-seq performed on cancer biopsies from patients treated with anti-CTLA-4 antibody (ipilimumab) before or after treatment with anti-PD-1 antibody (nivolumab) demonstrated that a major response to anti-CTLA-4 requires cancer cells with high levels of MHC-I expression at baseline, whereas the response to anti-PD-1 was more strongly associated with a pre-existing interferon-γ gene expression signature [88].