Another preclinical study conducted in mice suggests that performing treadmill endurance exercise decreases VEGF-A expression, as assessed through Enzyme-linked immunosorbent assay (ELISA) from tissue samples [100], while a rat model of a mammary tumor confirms an increased VEGF-A expression, detected immunohistochemically, and tumor vascularization after long-term training [101]. Here, VEGFA is linked to neoplasm.