In the univariate analysis, age at diagnosis ≥ 4 years, an unfavorable five-item microscopic score, tumor spillage, incomplete tumor resection, a tumor volume of ≥ 200 mL, a Ki67 index of >15%, and COG stages III/IV significantly impacted the negative prognostic factors upon the OS and EFS (Figure 3, Table 3). The gene discussed is MKI67; the disease is neoplasm.