To establish a biomarker panel of plasma-derived EVs for CRC detection, we used logistic regression to process the quantitative results acquired for the 5 targets (ADAM10, APMAP, CD58, CD59 and TSPAN9) that could be quantified in more than 50% (37 cases) of CRC samples with average concentrations higher than their respective LLOQ values in 153 subjects from HC (n = 80) and CRC (n = 73) groups. This evidence concerns the gene CD58 and colorectal carcinoma.