We found 11 tetraspanins (TSPAN1, TSPAN5, TSPAN6, TSPAN8, TSPAN9, TSPAN14, TSPAN15, CD9, CD81, CD82, and CD151) in the EV proteome of at least three CRC cell lines and detected CD63 in the EV proteome of Colo205 and SW620 cell lines (Tables S2 and S3). Here, TSPAN1 is linked to colorectal carcinoma.