A candidate biomarker list was then generated by integrated analysis of the 2 datasets, and tier 1 candidates containing 13 EV membrane proteins and the single EV marker, CD9, were selected for verification by targeted MS using product ion scanning (PIS) mode in 153 individual plasma EV samples isolated from 73 CRC patients and 80 HCs. The gene discussed is CD9; the disease is colorectal carcinoma.