In addition, PCR revealed a significant difference in the PGSC marker genes expression between diffuse gliomas, IDH-mut, grade 3, and astrocytomas, IDH-mut, grade 4, as well as oligodendrogliomas, grade 3, with signs of further malignancy oligodendrogliomas, IDH-mut, grade 3, with no signs of malignancy from the previous stage (p < 0.001, Figure 3d), and a significant difference between the same groups in terms of MES-like cell population driver genes (p < 0.001, Figure 3e). The gene discussed is IDH1; the disease is astrocytoma (excluding glioblastoma).