To perform a functional interpretation of our transcriptomic analyses, the DEGs genes resulting from each comparison between HER2+ and HER2− CRC patients, HER2+ and control CRC cell lines, and HER2+ and normal colon cell lines were initially used as the input for the GSEA to identify the significantly enriched pathways (p < 0.05) among gene sets related to the following: C2, C5 (BP and MF), C6, and C7 collections. This evidence concerns the gene ERBB2 and colorectal carcinoma.