Finally, we assessed the 2D invasiveness of colon cancer cells under the pharmacological inhibition of NaV1.5 channels using TTX, of NHE exchangers using EIPA, in cells transfected with irrelevant small interfering RNA (siCTL), or targeted to SCN5A (siSCN5A) or SLC9A1 (siSLC9A1). This evidence concerns the gene SCN5A and malignant colon neoplasm.