Finally, in order to investigate the anti-invasive activity of these two small-molecule drugs on colon cancer cells, we performed 2D invasion assays using Matrigel-coated cell culture inserts on SW620 and HCT116 cells stably expressing a short hairpin RNA targeting SCN5A gene expression (shNaV1.5) or a null-target shRNA (shCTL). This evidence concerns the gene SCN5A and malignant colon neoplasm.