TRIM25 and cancer: To further evaluate the impact of TRIM25 on caspase-7 mRNA decay, 24 h after the siRNA transfection the transcription in the RKO (A) or HCT-15 (B) cells was stalled by the addition of actinomycin D. Intriguingly, in both cancer cell lines, although at different particular time points after the blockade of the transcription, the remaining level of caspase-7 transcripts was significantly elevated upon TRIM25 attenuation (lower panels of Figure 2A,B), indicating that TRIM25 inhibits caspase-7 mRNA expression through a decrease in caspase-7 mRNA stability.