Western blot assay revealed that TSAIII significantly increased the expression of cleaved-caspase-3 (c-caspase-3), cleaved-caspase-9 (c-caspase-9), and cleaved-caspase-PARP (c-PARP) in both glioma cell lines (Figure 2B, p < 0.01 as compared to control). This evidence concerns the gene CASP9 and central nervous system cancer.