The commonly used tumor markers in clinical diagnosis of ESCC include carcinoembryonic antigen (CEA), carbohydrate antigen 19-9 (CA19-9), cytokeratin 19 fragment (CYFRA21-1), neuron specific enolase (NSE), squamous cell carcinoma antigen (SCC), Dickkopf-related protein 1 (DKK1) and so on. The gene discussed is SERPINB3; the disease is esophageal squamous cell carcinoma.