To further validate that hsa-mir-497_25g repressed OPA1 and VAPB, we transfected p497_25g into two glioma cell lines, i.e., U-118-MG and Hs683, and examined the expression levels of OPA1 and VAPB with quantitative RT-PCR (qRT-PCR) experiments. The gene discussed is OPA1; the disease is glioma.