The results of the Ct values (ranged from 23 to 25) indicated that the transcript abundance of myogenin in different tissues was high [26] (Cts < 29 are strong positive reactions indicative of abundant target nucleic acid in the sample, Cts of 30–37 are positive reactions indicative of moderate amounts of target nucleic acid, Cts of 38–40 are weak reactions indicative of minimal amounts of target nucleic acid, which could represent an infection state or environmental contamination). Here, MYOG is linked to infection.