Then, we drew a heatmap for the differential expression of these 5 ubiquitination-related genes, and as shown in Fig. 6B, the expression levels of UBE2S in tumor tissues were significantly higher than those in normal tissues, and the expression levels of ASB2, TRIM9, FBXO40, and EBF2 were higher in normal tissues than in tumor tissues. This evidence concerns the gene EBF2 and neoplasm.