Here, we investigated the altered expression of NEK9, EG5, and acetyl-α-tubulin, and their correlation with the EMT-related proteins (E-cadherin, claudin-1, vimentin, and β-catenin) to evaluate their role and possible prognostic significance for metastasis in patients with pathologic T3 stage (pT3) colon cancers. This evidence concerns the gene NEK9 and malignant colon neoplasm.