The PRPF8 mRNA level, as determined by qPCR, was significantly higher in HCC samples than in nontumor adjacent tissues (NTATs) in the retrospective cohort (Fig. 1a), and this pattern was corroborated in silico in the TGCA cohort (RNAseq data) (Fig. 1a). Here, PRPF8 is linked to hepatocellular carcinoma.