In GO enrichment analysis based on two open datasets, we observed the effects of Cmip on four genes, namely, Pparγ, Cebpb, Pexl1a, and Ccnd1, with the highest expression levels in mice fed an HFD; however, our data clearly showed that knockdown of Cmip interrupted Pparγ expression, suggesting that Cmip is responsible for the development of NAFLD. Here, CEBPB is linked to metabolic dysfunction-associated steatotic liver disease.