Similarly, we observe increased expression disease associated astrocyte51 (DAA) marker genes in the ALS-Glia subtype, including ITIH3, KCNIP4, PDGFD, ST6GALNAC5, and TNC. Interestingly, ALS-Glia expression of DAA genes suggests the astrocyte population in these patients captures both disease-associated and homeostatic phenotypes when compared to healthy control donors. Here, TNC is linked to amyotrophic lateral sclerosis.