AKT1 and infection: The strict requirement of Notch signaling for hepatocyte reprogramming and iCCA formation was confirmed in other studies utilizing R26RYFP–based, AAV8-Tbg-Cre infection-initiated hepatocyte lineage tracing combined with tumorigenesis triggered by HDTV injection of activated forms of AKT (myr-AKT) and Yap (YapS127A) oncogenes [61].