We used ELISA to detect the expression of GSDMB protein in the skin of 78 participants (21 PV patients, 57 normal subjects) and Western blot to detect the expression of GSDMB protein in the skin of 24 participants (12 PV patients, 12 normal subjects) (Tables 2 and 3). This evidence concerns the gene GSDMB and acquired polycythemia vera.