The results showed that compared with the control group, the contents of tumor necrosis factor- α, IL-6, IL-1β, 5-hydroxytryptamine, glutamicacid and ACH in the brain and serum of insomnia rats in the Jiaotai Pill group changed significantly.[27–29] “Jiaotai Pill” is often used to treat depression and insomnia in clinic. Here, TNF is linked to depressive disorder.