The results of GSEA showed that multiple inflammation-related pathways were significantly enriched in the liver of NASH mice, such as Inflammatory response (NES=1.83, FDR q<0.001), Interferon alpha response (NES=1.72, FDR q<0.001), Interferon gamma response (NES=1.80, FDR q<0.001), TNFα signaling via NFκB (NES=1.64, FDR q<0.001) and IL6-JAK-STAT3 (NES=1.79, FDR q<0.001) (Figure 4A). Here, TNF is linked to metabolic dysfunction-associated steatohepatitis.