After local injection of the EPI gel, the intratumoral proportion of CD4+CD25+Fox3+ cells (regulatory T cells, Tregs) in the right tumor was increased, but that in the left tumor was decreased; moreover, the CD3+CD8+ T lymphocytes in the right tumor were slightly reduced, but with no significant difference (Supplementary Figures S1,S2). Here, CD4 is linked to neoplasm.