In addition, the flow cytometry results showed that, on day 4 after local injection of the EPI gel, the expression of PD-L1 in the left tumor was upregulated, while that in bilateral tumors in mice was significantly reduced after anti-PD-L1 treatment, suggesting that local injection of the EPI gel may have promoted immunotherapy through the exposure of more PD-L1 to the anti-PD-L1 mAb. The gene discussed is CD274; the disease is neoplasm.