GFAP could not differentiate stroke mimics (including SDH) from AIS shown by an AUC of 0.47 (95% CI 0.40–0.55) or stroke mimics (including SDH) from stroke and TIA with an AUC of 0.54 (95% CI 0.47–0.60). Here, GFAP is linked to androgen insensitivity syndrome.