To further verify the regulatory relationship between TRAF6 and the RIPK1-RIPK3-MLKL signaling axis, we performed a subcutaneous transplantation tumor model of high TRAF6 expressing cells through RIPK3−/− and MLKL−/− mice of C57BL/6 genetic background (Fig. S5a). The gene discussed is RIPK1; the disease is neoplasm.