In addition, silencing MED27 (as a potential melanoma target) leads to a decrease in iNOS expression by inhibiting the activity of a series of key proteins in the NF-κB signaling pathway and is accompanied by the inhibition of melanoma cell proliferation, induction of apoptosis and regulation of the cell cycle by changing the activity of the PI3K/AKT, MAPK/ERK and Bax/Cyto-C/Caspase-dependent apoptotic pathways (27). The gene discussed is AKT1; the disease is melanoma.