Further evidence of aggressive tumor growth of the WSHFD-EL4 mice was showed by Western blot analysis of the protein levels of cyclin D1, c-Myc, and E-cadherin and Vimentin for epithelial–mesenchymal transition (EMT) in comparison with the CD-EL4 mice (Fig. 2G). This evidence concerns the gene CCND1 and neoplasm.