After 16 weeks WSHFD-feeding, significantly increased serum triglycerides, body weight, and adipose tissue weight along with the biomarkers of metabolic disorders (increased phosphorylation of hormone-sensitive lipase, adipocyte size, and inflammatory infiltration, but decreased insulin receptor substrate 1) were found in the WSHFD mice, with statistical significances compared to the control diet (CD) feeding (Fig. S1). The gene discussed is IRS1; the disease is metabolic disease.