Further analysis for S1P cascade synthetic enzymes (including Sptlc1, Cers [1–6], Des [1, 2], Acer [1–3], Asah1, Smase, Sms1, Cerk, Cert, Gcs, SphK1, SphK2, Spp1, and Sgpl1) showed that most enzymes were upregulated in the tumor tissues of EL4-WSHFD mice (Fig. 3B) and the result of fold changes (WSHFD-EL4 vs CD-EL4) revealed upregulation of those enzymes for the biosynthesis of sphinganine, sphingosine, ceramide and S1P(Fig. 3C). This evidence concerns the gene SPHK2 and neoplasm.