A next-generation sequencing (NGS) assay was developed assessing genes that are known to be frequently mutated or deleted in pancreatic cysts (KRAS, GNAS, NRAS, HRAS, BRAF, CTNNB1, TP53, PIK3CA, PTEN and AKT1) in combination with Sanger sequencing of VHL. This evidence concerns the gene PIK3CA and Pancreatic cysts.