Next, to investigate whether the seed region mutation in miR-3144-3p is directly catalyzed by ADAR1, we prepared two different plasmids, pcDNA3.1_ADAR1-p110_wild (the active functional form of ADAR1-p110 in liver cancer) and pcDNA3.1_ADAR1-p110_mutant (the form carrying a mutation in the adenine deaminase domain) (Supplementary Fig. 4b). This evidence concerns the gene ADAR and liver cancer.